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KMID : 0613820040140040614
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Journal of Life Science
2004 Volume.14 No. 4 p.614 ~ p.619
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Nucleus-DNA Damage and Different Response of Plant Cells to Paraquat in Relation to Enzyme Activity of Superoxide Dismutase
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Kwon Soon-Tae
Lee Myung-Hyun
Oh Sei-Myoung
Jung Do-Chul
Kim Kil-Ung
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Abstract
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This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/g·fresh weight and 547 unit/mg·protein, respectively, and those in L. japonica were 23 unit/g·fresh weight and 13 unit/mg·protein, respectively. SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.
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KEYWORD
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Comet assay, DNA damage, oxidative stress, SOD activity, cultured chlorophyllous cells
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